Consider the following reaction at equilibrium: CO2 + H2O ⇔ H2CO3. What would be the effect of adding additional H2O?

#Question id: 14271

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of cells at quasi-steady state when t = 10 h.

#Question id: 14272

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. If qP = 0.05 g product/g cells h and P₀ = 0.1 g/l, determine the product concentration in the vessel at t = 10 h

#Question id: 14273

#Section 5: Bioprocess Engineering and Process Biotechnology

Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is a stirred tank, containing initially 100 litres medium. The maximum specific growth rate of the culture is 0.18 d^-1 and the yield of biomass from substrate is 0.5 g g^-l. The concentration of growth-limiting substrate in the medium is 3% (w/v). The reactor is inoculated with 1.5 g l^{- 1} cells and operated in batch until the substrate is virtually exhausted; medium flow is then started at a rate of 4 l d^-1. Fed-batch operation occurs under quasi-steady-state conditions. Estimate the batch culture time.                                  ____________________

#Question id: 14274

#Section 5: Bioprocess Engineering and Process Biotechnology

Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is a stirred tank, containing initially 100 litres medium. The maximum specific growth rate of the culture is 0.18 d^-1 and the yield of biomass from substrate is 0.5 g g^-l. The concentration of growth-limiting substrate in the medium is 3% (w/v). The reactor is inoculated with 1.5 g l^{- 1} cells and operated in batch until the substrate is virtually exhausted; medium flow is then started at a rate of 4 l d^-1. Fed-batch operation occurs under quasi-steady-state conditions. Estimate final biomass concentration?

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