The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction:

Determine the product yield coefficients YC3H8O3/S  _____________

TLS Online TPP Program

#Question id: 12904

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

1. Southern Blotting	i. Melting temperature of DNA strand
2. DNA microchips	ii. oligonucleotide matching to DNA sample
3. ASO-PCR	iii. DNA hybridization to oligo arrays
4. DGGE	iv. labeled probe hybridization to DNA

TLS Online TPP Program

#Question id: 12905

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Some gene/protein is used to identify the Disorder in humans Match them correctly:

1. Tyrosine Kinase(TK)	i. Atherosclerosis
2. HIV transactivator	ii. Hypertension
3. Angiotensinogen	iii. Kaposis Sarcoma
4. CET protein	iv. Cardiac Hypertrophy

TLS Online TPP Program

#Question id: 12906

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

An alternative method labeling strategy used in karyotyping and gene localisation is _______

TLS Online TPP Program

#Question id: 12907

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____

TLS Online TPP Program

#Question id: 12908

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which statement is incorrect about CRISPR?

TLS Online TPP Program

#Question id: 12909

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.