Changes in the topology and three-dimensional shape of B-DNA can result from

TLS Online TPP Program

#Question id: 12936

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Adding small double-stranded molecules with one internal site for a restriction endonuclease,termed ______ to cDNA.

TLS Online TPP Program

#Question id: 12937

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the incorrect statement about Yeast Artificial Chromosome vectors.

TLS Online TPP Program

#Question id: 12938

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

What is the main advantage of using Yeast Artificial Chromosome based vector over other vectors?

1. It has ability to clone very large fragments of DNA.

2. YAC vectors do not contain origin of replication sites 

3. It has insert  range up to 2000 kb.

4. It help in genome mapping and sequencing projects.

TLS Online TPP Program

#Question id: 12939

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the incorrect statement for the production of site-directed mutations.

TLS Online TPP Program

#Question id: 12940

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Choose the correct order for the steps involve in Protein engineering.

1. Protein modelling

2. Protein crystallography

3. Site-directed mutations

4. Comparison with entries in databases of known proteins.

TLS Online TPP Program

#Question id: 12941

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the therapeutic protein which has been used to successfully treat certain types of breast cancer.