TLS Online TPP Program
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TLS Online TPP Program
#Question id: 14362
#Section 5: Bioprocess Engineering and Process Biotechnology
An experiment requires the use of 5 x 107 units of enzyme. The price for the enzyme is quoted for katal amounts. Allowing for a 20% excess for wastage, pipetting, and so forth, what approximate round number of katals of enzyme would you purchase?
TLS Online TPP Program
#Question id: 14363
#Section 5: Bioprocess Engineering and Process Biotechnology
Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate the specific activities in units/g at each of the two steps
TLS Online TPP Program
#Question id: 14364
#Section 5: Bioprocess Engineering and Process Biotechnology
Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate % increase in activity obtained over the two step
TLS Online TPP Program
#Question id: 14365
#Section 5: Bioprocess Engineering and Process Biotechnology
0.1 mg of a quaternary structured enzyme (Mr 486 000) with four active sites converts 48 iimol substrate/10 sec. Calculate turnover number (min-1)
TLS Online TPP Program
#Question id: 14366
#Section 5: Bioprocess Engineering and Process Biotechnology
0.1 mg of a quaternary structured enzyme (Mr 486 000) with four active sites converts 48 mirormol substrate/10 sec. Calculate molecular activity (min^-1), catalytic centre activity (min^-1). -
TLS Online TPP Program
#Question id: 14367
#Section 5: Bioprocess Engineering and Process Biotechnology
During an enzyme purification, 0.23 g of protein was dissolved in 5 mL buffer. Suppose 0.5 mL of this solution converted 4.7 mmol of substrate in 30 sec. Calculate specific activity as units/mg protein -