TLS Online TPP Program

#Question id: 645


Which of the following is the correct combination of marker enzymes used to identify different organelles during subcellular fractionation of eukaryotic tissue?

#Section 2: General Biology
  1. Cytosol-Catalase; Mitochondria-Succinate Dehydrogenase; Lysosome-Acid phosphatase; Peroxisome-Lactate Dehydrogenase

  2. Cytosol-Acid phosphatase; Mitochondria-Succinate Dehydrogenase; Lysosome-Lactate Dehydrogenase; Peroxisome-Catalase 

  3. Cytosol-Lactate Dehydrogenase; Mitochondria-Succinate Dehydrogenase; Lysosome-Acid phosphatase; Peroxisome-Catalase

  4. Cytosol-Succinate Dehydrogenase; Mitochondria-Lactate Dehydrogenase; Lysosome-Acid phosphatase; Peroxisome-Catalase

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TLS Online TPP Program

#Question id: 14356

#Section 5: Bioprocess Engineering and Process Biotechnology

 In Luedeking—Piret equation, if = 0 then product is 

TLS Online TPP Program

#Question id: 14357

#Section 5: Bioprocess Engineering and Process Biotechnology

Milk is pasteurized if it is heated to 630C for 30 min, but if it is heated to 74°C it only needs 15 s for the same result. Find the activation energy of this sterilization process.  J/mol 

TLS Online TPP Program

#Question id: 14358

#Section 5: Bioprocess Engineering and Process Biotechnology

One liter per minute of liquid containing A and B (CA0 = 0.10 mollliter, CB() = 0.01 mollliter, Cco=0) flow into a mixed reactor of volume V = 1 liter. The materials react in a complex manner for which the stoichiometry is unknown. The outlet stream from the reactor contains A, B, and C (CAf = 0.02 mollliter, CBf = 0.03 mollliter, Cci = 0.04 mollliter), as shown in fig. 


Find the rate of reaction of A, B, and C for the conditions within the reactor  mol/liter.min 

TLS Online TPP Program

#Question id: 14359

#Section 5: Bioprocess Engineering and Process Biotechnology

How many enzyme units are there in 1 katal? 

TLS Online TPP Program

#Question id: 14360

#Section 5: Bioprocess Engineering and Process Biotechnology

1 pg of an enzyme (Mr 160 000) converts 0.192 pmol/min. This enzyme is made up of four subunits. Calculate turnover number 

TLS Online TPP Program

#Question id: 14361

#Section 5: Bioprocess Engineering and Process Biotechnology

1 m of an enzyme (Mr 160 000) converts 0.192 i.imol/min. This enzyme is made up of four  subunits. Calculate catalytic centre activity