#Question id: 13071
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
#Question id: 13072
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
#Question id: 13073
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
#Question id: 13074
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
#Question id: 13075
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
#Question id: 13076
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Restriction fragment length polymorphism denotes that a
single restriction enzyme produces fragments of different lengths from the same
stretch of genomic DNA of different strains of a species or from different
related species. RFLPs are detected as follows:
i. Large
molecular weight genomic DNA is isolated from several strains or related
species;
ii. The
fragments in these digests are separated through electrophoresis
iii. These
'DNAs are then digested with a selected restriction enzyme
iv. Exposed
to a suitably radio-labelled appropriate DNA probe under conditions favouring
DNA: DNA hybridization
v. The
resulting gel lanes are transferred and fixed to a suitable solid support and
vi. The free
probes are removed
vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.