TLS Online TPP Program

#Question id: 4347


According to the lac operon model proposed by Jacob and Monod, what is predicted to occur if the operator is removed from the operon?

#Section 3: Genetics, Cellular and Molecular Biology
  1. The lac operon would be transcribed continuously.

  2. Only lacZ would be transcribed.

  3. Only lacY would be transcribed.

  4. Galactosidase permease would be produced, but would be incapable of transporting lactose.

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TLS Online TPP Program

#Question id: 14622

#Section 2: General Biology

Endospores are

TLS Online TPP Program

#Question id: 14623

#Section 5: Bioprocess Engineering and Process Biotechnology

a chromatographic separation of a two component samples on 50 cm column gave the retention times for the solutes A and B as 2.5 and 3.1 minutes with base widths of the two chromatographic peaks being 0.24 and 0.3 min respectively. Calculate resolution of two peak 

TLS Online TPP Program

#Question id: 14624

#Section 5: Bioprocess Engineering and Process Biotechnology

An enzyme is produced for manufacturing a sun protection lotion. Given kinetic data for the enzyme reaction with vmax= 2.5mmol M-3 S-1 Km= 8.9 mM and So = 12 mM, what would be the time required for 95% conversion in a batch bioreactor? 

TLS Online TPP Program

#Question id: 14625

#Section 5: Bioprocess Engineering and Process Biotechnology

Monod kinetics are considered in a CSTR with an organism growing with an initial substrate concentration of 50 g1-1 and kinetic parameters of Ks = 2 g1-1 and umax = 0.5 h-1 . (a) What would be the maximum dilution rate for 100% yield of biomass with maximum rate? 

TLS Online TPP Program

#Question id: 14626

#Section 5: Bioprocess Engineering and Process Biotechnology

A bacterial fermentation was carried out in a reactor containing broth with average density 1200 kg/m3 and viscosity 0.02 N.s/m2 . The broth was agitated at 90 rpm and air was introduced through the sparger at a flow rate of 0.4 vvm. The fermenter was equipped with two sets of flat blade turbine impellers and four baffles. The dimensions of vessel, impellers and baffle width were: tank diameter, Dt =4 m; impeller diameter, Di = 2 m; baffle width, Wb = 0.4 m; also, the liquid depth was H = 6.5 m 
Determine ungassed power, P; 

TLS Online TPP Program

#Question id: 14627

#Section 5: Bioprocess Engineering and Process Biotechnology

In a batch production of penicillin, 40 m3 capacity of the plant, sterile air is required to be supplied. The bioreactor requires 1 vvm (volume of air/volume of broth. min). The incoming air contains 3000 cells/m3of air, for 100 hours operation. Calculate the depth of filter if the penetration of bacteria is 1 in 1 million.