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TLS Online TPP Program

#Question id: 13107

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.
 

 
Why is it that some people show two bands?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. The more repeats you have, the longer your DNA fragment is, and longer DNA fragments migrate more slowly in gels.
  2. They are homozygotes for the allele that cannot be cut.
  3. They are homozygotes for the allele that can be cut.
  4. They are heterozygotes, and possess one allele that can be cut and one allele that cannot be cut.
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TLS Online TPP Program

#Question id: 14625

#Section 5: Bioprocess Engineering and Process Biotechnology

Monod kinetics are considered in a CSTR with an organism growing with an initial substrate concentration of 50 g1-1 and kinetic parameters of Ks = 2 g1-1 and umax = 0.5 h-1 . (a) What would be the maximum dilution rate for 100% yield of biomass with maximum rate? 
TLS Online TPP Program

#Question id: 14626

#Section 5: Bioprocess Engineering and Process Biotechnology

A bacterial fermentation was carried out in a reactor containing broth with average density 1200 kg/m3 and viscosity 0.02 N.s/m2 . The broth was agitated at 90 rpm and air was introduced through the sparger at a flow rate of 0.4 vvm. The fermenter was equipped with two sets of flat blade turbine impellers and four baffles. The dimensions of vessel, impellers and baffle width were: tank diameter, Dt =4 m; impeller diameter, Di = 2 m; baffle width, Wb = 0.4 m; also, the liquid depth was H = 6.5 m 
Determine ungassed power, P; 
TLS Online TPP Program

#Question id: 14627

#Section 5: Bioprocess Engineering and Process Biotechnology

In a batch production of penicillin, 40 m3 capacity of the plant, sterile air is required to be supplied. The bioreactor requires 1 vvm (volume of air/volume of broth. min). The incoming air contains 3000 cells/m3of air, for 100 hours operation. Calculate the depth of filter if the penetration of bacteria is 1 in 1 million. 
TLS Online TPP Program

#Question id: 14628

#Section 5: Bioprocess Engineering and Process Biotechnology

Microbial cells are separated from a culture broth at a flow rate of 3.35 x10-3m3/5. Assume the cells are spherical with average diameter of 111m. Select a centrifuge that can perform this separation. Given data: pcell =1.1, Pwater,=997 kgm-1 at 25 °C; Pbroth = 3pwater, and the viscosity of water is 0.9 x 10-3 N.s/m2 
TLS Online TPP Program

#Question id: 14629

#Section 5: Bioprocess Engineering and Process Biotechnology

Microbial cell recovery is carried out in a continuous disc-stack centrifuge. The centrifuge is operated at 5000 rpm for separation of baker's yeast. At a feed rate of 60 1/min, 50% of the cells are recovered. At constant speed, solid recovery is inversely proportional to flow rate. What flow rate is required to recover 90% of cells if the centrifuge speed is fixed at 5000 rpm? 
TLS Online TPP Program

#Question id: 14630

#Section 1: Engineering Mathematics

The series whose nth term is sin 1/n is