Microbial cells are separated from a culture broth at a flow rate of 3.35 x10^-3m3/5. Assume the cells are spherical with average diameter of 111m. Select a centrifuge that can perform this separation. Given data: p_cell =1.1, P_water,=997 kgm^-1 at 25 °C; P_broth = 3p_water, and the viscosity of water is 0.9 x 10^-3 N.s/m² 

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#Question id: 13078

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

RFLPs have several unique advantages except this one;

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#Question id: 13079

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,

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#Question id: 13080

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In RAPDs amplification will take place only of those regions of the genome that have the 

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#Question id: 13081

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Correct statements about RAPD’s

A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.

B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,

C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.

D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.

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#Question id: 13082

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Statement: AFLP shares some features of both RFLP and RAPD analyses.

Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

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#Question id: 13083

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 

(1) both ends cleaved by PstI (Pst-Pst), 

(2) both ends cleaved by MseI (Mse-Mse), and 

(3) one end generated by each of the two enzymes (Pst-Mse). 

the most frequent fragments in decreasing order,