TLS Online TPP Program

#Question id: 14628


Microbial cells are separated from a culture broth at a flow rate of 3.35 x10-3m3/5. Assume the cells are spherical with average diameter of 111m. Select a centrifuge that can perform this separation. Given data: pcell =1.1, Pwater,=997 kgm-1 at 25 °C; Pbroth = 3pwater, and the viscosity of water is 0.9 x 10-3 N.s/m2 

#Section 5: Bioprocess Engineering and Process Biotechnology
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TLS Online TPP Program

#Question id: 14279

#Section 5: Bioprocess Engineering and Process Biotechnology

Growth yield of mDicrobes is defined as

TLS Online TPP Program

#Question id: 14280

#Section 5: Bioprocess Engineering and Process Biotechnology

In microbial cultivation, the expression for product synthesis

TLS Online TPP Program

#Question id: 14281

#Section 5: Bioprocess Engineering and Process Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 


if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 

TLS Online TPP Program

#Question id: 14282

#Section 5: Bioprocess Engineering and Process Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the

TLS Online TPP Program

#Question id: 14283

#Section 5: Bioprocess Engineering and Process Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

TLS Online TPP Program

#Question id: 14284

#Section 5: Bioprocess Engineering and Process Biotechnology

The specific productivity (qp) of an enzyme production Is fitted linearly with specific growth rate p of a fungal organism according to the equation The estimated values of constants alpha and beta are 0.0006 and 25 respectively. The enzyme production kinetics is