TLS Online TPP Program

#Question id: 13098


You are a scientist who is using genomics to currently study a new bacterial species that no one has ever studied before. The following sequence is a piece of DNA within the coding region of a gene that you have recently sequenced.
 
How many of the 6 potential open reading frames are actually open in this sequence shown above?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. There is only 2 reading frame
  2. There is only 3 reading frame
  3. There is only 1 reading frame
  4. There is no open reading frame actually in these sequence
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TLS Online TPP Program

#Question id: 14166

#Section 5: Bioprocess Engineering and Process Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                             

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium? 

TLS Online TPP Program

#Question id: 14167

#Section 4: Fundamentals of Biological Engineering

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

 How much water is required?


TLS Online TPP Program

#Question id: 14167

#Section 5: Bioprocess Engineering and Process Biotechnology

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

 How much water is required?


TLS Online TPP Program

#Question id: 14168

#Section 4: Fundamentals of Biological Engineering

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

What is the concentration of solids in the final mixture?

TLS Online TPP Program

#Question id: 14168

#Section 5: Bioprocess Engineering and Process Biotechnology

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

What is the concentration of solids in the final mixture?

TLS Online TPP Program

#Question id: 14169

#Section 4: Fundamentals of Biological Engineering

Unsweetened condensed milk is a shelf-stable canned milk product. If 100kg of fresh milk, containing 87% water and the rest milk solids, is condensed using an evaporator to remove 60% of the water from the fresh milk, calculate:

 The mass of water evaporated