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TLS Online TPP Program

#Question id: 19486

The growth of S.cerevisiae on glucose under anaerobic conditions can be described by the following reaction:
C6H12O6 + aNH3 → 0.59 CH1.64N0.16O0.52 (biomass) + 0.43 C3H8O3 + 1.54CO2 + 1.3C2H5OH + 0.036H2O 
and determine the product yield coefficient Y etoh/s and determine coefficient a.

#Section 4: Fundamentals of Biological Engineering
  1. 0.57 g. g⁻¹, 0.078
  2. 0.33 g. g⁻¹, 0.094
  3. 0.45 g. g⁻¹, 0.086
  4. 0.44 g. g⁻¹, 0.056
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TLS Online TPP Program

#Question id: 13091

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
Which colony’s plasmid do you actually want to use for your studies?
TLS Online TPP Program

#Question id: 10536

#Section 6: Plant, Animal and Microbial Biotechnology

Hormonal interactions contribute to plant–insect herbivore interactions such as— ethylene, salicylic acid, and jasmonic acid. In particular, ethylene appears to play an important role in this context, what will be the effect of ethylene when is applied alone and when is applied with other amino acids?

a) ethylene applied together with JA it seems to reduces defense responses

b) Ethylene appears to applied alone to plants, ethylene has little effect on defense-related gene activation

c) when ethylene applied together with JA it seems to enhance JA responses or defense responses are significantly increased

d) Ethylene appears to applied alone to plants, ethylene has more effect on defense-related gene activation

Which of the following combination is correct about ethylene effects?
TLS Online TPP Program

#Question id: 2107

#Section 3: Genetics, Cellular and Molecular Biology

All the following statements describe biomembranes except:
TLS Online TPP Program

#Question id: 19144

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

As the E value of a BLAST search becomes smaller 
TLS Online TPP Program

#Question id: 14166

#Section 5: Bioprocess Engineering and Process Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                             

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium?