To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 3’s plasmid is;

#Question id: 10148

#Section 2: General Biology

How light controls the assembly of chloroplast enzymes into supramolecular complexes?

a.) Light regulates the stability of the ternary complex through the ferredoxin–thioredoxin system

b.) Reduced thioredoxin cleaves the disulfide bonds of both phosphoribulokinase and CP12, releasing glyceraldehyde-3-phosphate dehydrogenase and phosphoribulokinase in the catalytically active conformations

c.) Oxidised thioredoxin cleaves the disulfide bonds of both phosphoglyceratekinase and CP12, releasing glyceraldehyde-3-phosphate dehydrogenase and phosphoglyceratekinase in the catalytically active conformations

d.) thioredoxin system oxidised the disulfide bonds and reduces the assembly of between chloroplast enzymes and CP12 and converted into the catalytically active conformations

Which of the following statement is correct?

#Question id: 10150

#Section 2: General Biology

Light controls the assembly of chloroplast enzymes into supramolecular complexes one of the enzyme such as Glyceraldehyde-3-P dehydrogenase, this enzyme acivity is dependent on

#Question id: 10153

#Section 2: General Biology

Which of the following statements is False?

#Question id: 10155

#Section 2: General Biology

Which of the following components carries out oxygen cycle and also produces hydrogen per oxide ?

#Question id: 10156

#Section 2: General Biology

Which of the following statements is False about Rubisco?

#Question id: 10157

#Section 2: General Biology

In Glycolate pathway, Glycine exits the peroxisomes and enters the mitochondria where a multienzyme complex catalyzes the conversion of two molecules of glycine and one molecule of NAD+ into one molecule each of serine, NADH, NH4 +, and CO2; the enzyme complex used in this reaction is