Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 3863

#Section 3: Genetics, Cellular and Molecular Biology

Introns in nuclear pre-mRNAs are excised by:

TLS Online TPP Program

#Question id: 3864

#Section 3: Genetics, Cellular and Molecular Biology

RNA polymerase:

TLS Online TPP Program

#Question id: 3865

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following statements about E. coli RNA polymerase is false?

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#Question id: 3866

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following statements about E. coli RNA polymerase (core enzyme) is false?

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#Question id: 3867

#Section 3: Genetics, Cellular and Molecular Biology

RNA polymerase from E. coli (core enzyme alone) has all of the following properties except that it:

TLS Online TPP Program

#Question id: 3868

#Section 3: Genetics, Cellular and Molecular Biology

The sigma factor of E. coli RNA polymerase: