Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 14912

#Section 1: Engineering Mathematics

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#Question id: 3527

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following genetic cross would produce 1/4 yellow round, 1/4 yellow wrinkle, 1/4 Green round and ¼ Green Wrinkle seeded plant?

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#Question id: 14631

#Section 1: Engineering Mathematics

The series 

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#Question id: 1120

#Section 3: Genetics, Cellular and Molecular Biology

A mutation that knocks out the GTPase activity of a G protein would have what effect on a cell?

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#Question id: 15570

#Section 1: Engineering Mathematics

To solve x² - 2 = 0, the Newton-Raphson method has been employed. If the initial guess xo = 1.0, the next estimate of the root, x1, will be