Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

#Question id: 27518

#Section 5: Bioprocess Engineering and Process Biotechnology

In a fermentation process, air sterilizer is used to reduce 1×108 to 1 microorganisms. The filtration efficiency will be

#Question id: 27519

#Section 5: Bioprocess Engineering and Process Biotechnology

The destruction of the cells takes place during following treatment processes

#Question id: 27520

#Section 5: Bioprocess Engineering and Process Biotechnology

In the air filter, the particles removal due to drag force

#Question id: 27521

#Section 5: Bioprocess Engineering and Process Biotechnology

In the figure below, '1', '2' and '3' stand for

#Question id: 27558

#Section 5: Bioprocess Engineering and Process Biotechnology

10 kg of a pure enzyme (Mr 30 000) catalyses the conversion of 1.5 g of a substrate (Mr 44) to products in 5 minutes. What will be the turnover number in (sec^-1)  ....10^5

#Question id: 27562

#Section 5: Bioprocess Engineering and Process Biotechnology

Match each parameter in Group 1 with the appropriate measuring device in Group 2

Group 1                                       Group 2

(P) Pressure                               (1) Photometer

(Q) Foam                                    (2) Rotameter

(R) Turbidity                               (3) Diaphragm gauge

S) Flow rate                                (4) Rubber sheathed electrode