Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 18088

#Section 1: Engineering Mathematics

If a coin is tossed 5 times, then consider the following statements:

I: Probability of getting exactly 2 heads is 5/16.

II: Probability of getting at least 4 heads is 3/16.

III: Probability of getting at most 2 heads is 5/8.

Which one of the following is correct in respect of the above statements

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#Question id: 18147

#Section 2: General Biology

Which of the following physical sterilization processes can denature proteins?

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#Question id: 18148

#Section 2: General Biology

Which is (are) true concerning the cell wall of prokaryotes? Which of the following are correct.

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#Question id: 18149

#Section 2: General Biology

The cell wall of Gram-negative organisms

Which combination is correct?

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#Question id: 18150

#Section 2: General Biology

Membrane phospholipids include

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#Question id: 18151

#Section 2: General Biology

The standard free-energy changes for the reactions below are given. Phosphocreatine -> creatine + Pi DG'° = –43.0 kJ/mol ATP ->  ADP + Pi DG'° = –30.5 kJ/mol What is the overall DG'° for the following reaction? Phosphocreatine + ADP -> creatine + ATP_______ kJ/mol