Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

#Question id: 3527

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following genetic cross would produce 1/4 yellow round, 1/4 yellow wrinkle, 1/4 Green round and ¼ Green Wrinkle seeded plant?

#Question id: 3528

#Section 3: Genetics, Cellular and Molecular Biology

Consider the following cross:

A/a ; B/b ; C/c ; D/d ; E/e  X a/a ; B/b ; c/c ; D/d ; e/e

What proportion of progeny will phenotypically resemble the first parent?

#Question id: 3529

#Section 3: Genetics, Cellular and Molecular Biology

Mendel crossed tall and round seeded pea plants with dwarf and wrinkle seeded pea. The F1 plants were all tall and round seed. What is the probability of tall plant with wrinkle seed in F2 generation?

#Question id: 3530

#Section 3: Genetics, Cellular and Molecular Biology

Mendel’s law of segregation, as applied to the behavior of chromosomes in meiosis, means that:

#Question id: 3531

#Section 3: Genetics, Cellular and Molecular Biology

Due to independent assortment resulting gamete is

#Question id: 3532

#Section 3: Genetics, Cellular and Molecular Biology

In a plant species, flower color purple is dominant over white. One such purple-flowered plant upon selfing produced 35 viable plants, of which 9 were white flowered and the rest were purple flowered. What fraction of these purple-flowered progeny is expected to be pure purple-flowered line?