Choose the correct order for the cloning process.

1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.

2. mRNA cannot be used directly in cloning.

3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.

4. The cDNA may be inserted into vectors and then cloned.

TLS Online TPP Program

#Question id: 27322

#Section 5: Bioprocess Engineering and Process Biotechnology

If the maintenance coefficient (m) is significantly high, then with reduction in specific growth rate:

TLS Online TPP Program

#Question id: 27323

#Section 5: Bioprocess Engineering and Process Biotechnology

In microbial cultivation experiment with cells having significant maintenance requirements, the measured  growth yield Y’ XS (G) and the true growth yield Y XS

TLS Online TPP Program

#Question id: 27324

#Section 5: Bioprocess Engineering and Process Biotechnology

During a batch culture experiment, the following data was obtained

What will be the product yield (g) per gram of substrate?

TLS Online TPP Program

#Question id: 27325

#Section 5: Bioprocess Engineering and Process Biotechnology

if the initial substrate concentration is 15 g/L and initial biomass concentration is 0.1 g/L. The substrate is then consumed completely and produces 6.4 g/L of biomass. The yield coefficient will be

TLS Online TPP Program

#Question id: 27326

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of a microorganism on glucose is described by the Monod model (µmax = 0.7 h¹ and Ks = 0.1 g L¹). If the initial biomass concentration in the batch fermenter is 0.1 g L¹, the minimum time required to triple the biomass concentration would be

TLS Online TPP Program

#Question id: 27327

#Section 5: Bioprocess Engineering and Process Biotechnology

The product formation kinetics of a biochemical process follows the model qp = au + B. In case of a = 0, the rate of product formation is