Arrange the steps of stages in PCR in correct order.

1. The region to be specifically amplified is made accessible within the complex DNA.

2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.

3. A thermal cycler is used to incrementally decrease the annealing temperature.

4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

TLS Online TPP Program

#Question id: 16030

#Section 6: Plant, Animal and Microbial Biotechnology

Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

TLS Online TPP Program

#Question id: 16031

#Section 6: Plant, Animal and Microbial Biotechnology

Which of the following is the technique used for the embryo culture?

TLS Online TPP Program

#Question id: 16032

#Section 6: Plant, Animal and Microbial Biotechnology

The major problem associated with the isolation of free cells and cell aggregates from organs is that of

TLS Online TPP Program

#Question id: 16072

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following is not a benefit of FASTA over BLAST?

TLS Online TPP Program

#Question id: 16073

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The initiation of FASTA format has ___ symbol.

TLS Online TPP Program

#Question id: 16074

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the items in Group 1 with an appropriate description in Group 2 

Group 1                                                    Group 2 

P. PSI-BLAST                                    1. ungapped, aligned motif 

Q. CLUSTALW                                 2. Profiles

R. BLOCK                                         3. Heuristic method

S. FASTA                                           4. Multiple sequence alignment