Arrange the steps of stages in PCR in correct order.

1. The region to be specifically amplified is made accessible within the complex DNA.

2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.

3. A thermal cycler is used to incrementally decrease the annealing temperature.

4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

#Question id: 2086

#Section 2: General Biology

Which of the following is (are) not present in biomembranes?

#Question id: 15686

#Section 1: Engineering Mathematics

if  than the matrix X which satisfies the  equation 3A + X = 2B is given by X = 

#Question id: 1966

#Section 2: General Biology

Mammals have Toll-like receptors (TLRs) that act in a manner similar to those of insects. While not specific to a particular pathogen, a TLR can recognize a kind of macromolecule that is absent from vertebrates but present in/on certain groups of pathogens. Which of the following is most likely to be recognized by a particular TLR that defends against some viruses?

#Question id: 14312

#Section 5: Bioprocess Engineering and Process Biotechnology

An industrial waste-water stream is fed to a stirred-tank reactor continuously and the cells are recycled back to the reactor from the bottom of the sedimentation tank placed after the reactor. The following are given for the system: F = 100 l/h; S0 = 5000 mg/l; mm = 0.25 h-1 ; Ks = 200 mg/l; α (recycle ratio) = 0.6; C (cell concentration factor) = 2; Y ^M _X/S = 0.4. The effluent concentration is desired to be 100 mg/l.  Determine the required reactor volume.

#Question id: 139

#Section 2: General Biology

 A sequence of amino acids with a relatively high hydropathy is very likely to function by