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TLS Online TPP Program

#Question id: 12908

Which statement is incorrect about CRISPR?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. It  was first identified as an adaptive immunity pathway in prokaryotes providing resistance to bacteriophage and analogous to the RNA interference process found in eukaryotes. 
  2. It does not utilize double-stranded sequence-specific breaks and does not  repair using HDR via homologous recombination.
  3. The system consists of two parts, a short synthetic guide RNA ( gRNA) and a nonspecific double-stranded endonuclease termed Cas9. 
  4. It is also possible to program Cas9 with multiple guide RNAs to allow multiplex site-specific editing for designing large deletions, inversions and translocations.
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TLS Online TPP Program

#Question id: 14172

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of baker’s yeast (S. cerevisiae) on glucose may be simply described by the following equation:
In a batch reactor of volume 105 l, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry. Determine the yield coefficients YX/S (biomass/glucose)?
TLS Online TPP Program

#Question id: 14173

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of baker’s yeast (S. cerevisiae) on glucose may be simply described by the following equation:
In a batch reactor of volume 10^5 l, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry. Determine the yield coefficient YX/O2 (biomass/oxygen)?
TLS Online TPP Program

#Question id: 14174

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of baker’s yeast (S. cerevisiae) on glucose may be simply described by the following equation:


In a batch reactor of volume 10^5 l, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry, Determine the total amount of oxygen required?

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TLS Online TPP Program

#Question id: 14175

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the biomass yield coefficient YX/S? 
TLS Online TPP Program

#Question id: 14176

#Section 5: Bioprocess Engineering and Process Biotechnology

 The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the product yield coefficient YEtOH/S 
TLS Online TPP Program

#Question id: 14177

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the product yield coefficients YCO2/S