Practice Questions

TLS Online TPP Program

#Question id: 12908

Which statement is incorrect about CRISPR?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

It was first identified as an adaptive immunity pathway in prokaryotes providing resistance to bacteriophage and analogous to the RNA interference process found in eukaryotes.
It does not utilize double-stranded sequence-specific breaks and does not repair using HDR via homologous recombination.
The system consists of two parts, a short synthetic guide RNA ( gRNA) and a nonspecific double-stranded endonuclease termed Cas9.
It is also possible to program Cas9 with multiple guide RNAs to allow multiplex site-specific editing for designing large deletions, inversions and translocations.

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TLS Online TPP Program

#Question id: 14624

#Section 5: Bioprocess Engineering and Process Biotechnology

An enzyme is produced for manufacturing a sun protection lotion. Given kinetic data for the enzyme reaction with v_max= 2.5mmol M^-3 S^-1 Km= 8.9 mM and So = 12 mM, what would be the time required for 95% conversion in a batch bioreactor?

TLS Online TPP Program

#Question id: 1275

#Section 3: Genetics, Cellular and Molecular Biology

The functions of the extracellular matrix include:

a. supporting differentiation.

b. inducing morphogenesis.

c. binding growth hormones.

d. repairing damaged tissue

which of the following combination is correct?

TLS Online TPP Program

#Question id: 14320

#Section 5: Bioprocess Engineering and Process Biotechnology

Both Saccharomyces cerevisiae yeast and Zymomonas mobilis bacteria produce ethanol from glucose under anaerobic conditions without external electron acceptors. The biomass yield from glucose is 0.11 g g-1 for yeast and 0.05 g g-I for Z. mobilis. In both cases the nitrogen source is NH 3. Both cell compositions are represented by the formula CH 1.800.5N0.2. What is the yield of ethanol from glucose by Zymomonas mobilis bacteria?

TLS Online TPP Program

#Question id: 2272

#Section 3: Genetics, Cellular and Molecular Biology

Bacteria may be propelled by

TLS Online TPP Program

#Question id: 2682

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

DNA fingerprinting recognizes the differences in