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TLS Online TPP Program
#Question id: 3262
#Section 3: Genetics, Cellular and Molecular Biology
In a population, the genotype frequencies are: f(A1A1)= 0.35; f(A1A2) = 0.30; f(A2A2) = 0.35; Which of the following statement is correct?
TLS Online TPP Program
#Question id: 14600
#Section 2: General Biology
The proteins of bacteria that are involved in the movement of small molecules into the cell are called
TLS Online TPP Program
#Question id: 14396
#Section 5: Bioprocess Engineering and Process Biotechnology
A purified hydrolytic enzyme (Mr 150 000) is available with the description: Gives a Vmax of 3 200 units/mg protein under optimal conditions. How many micromoles of substrate can be hydrolysed by 1 mg of protein in 1 minute
TLS Online TPP Program
#Question id: 13054
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program
#Question id: 4137
#Section 3: Genetics, Cellular and Molecular Biology
Formation of the ribosomal initiation complex for bacterial protein synthesis does not require: