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TLS Online TPP Program

#Question id: 13084

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. II is true, but not correct explanation of given statement.
  2. I and II both are true but not correct explanations of given statement.
  3. I is true and correct explanation of given statement.
  4. I and II both are true and correct explanations of given statement.
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TLS Online TPP Program

#Question id: 12919

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is not correct about RT-PCR. 
TLS Online TPP Program

#Question id: 12920

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The development of isothermal systems such as the ____ DNA amplification system do away with the need for a thermal cycler and have the advantage of being able to be used outside the laboratory.
TLS Online TPP Program

#Question id: 12921

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:-

A. Gene probe production.i. Novel proteins
B. Genome mapping.ii. PCR mutagenesis
C. Protein Engineering.iii. Sequence tagged sites
D. Gene Editing.iv. Use with blots
TLS Online TPP Program

#Question id: 12922

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Match the following:-

1. Ligase chain reaction.i. Mutation detection
2. Nucleic acid sequence based amplification.ii. Non isothermal
3. Loop mediated isothermal amplification.iii. Viral detection
4. Branched DNA amplification.iv. parasitic disease
TLS Online TPP Program

#Question id: 12923

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the technology which is used as a means of identifying the initial concentrations of DNA or cDNA template used.
TLS Online TPP Program

#Question id: 12924

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In order to quantify unknown DNA templates, a standard dilution is prepared using DNA of known concentration. As the DNA accumulates during the early exponential phase of the reaction,an arbitrary point is taken where each of the diluted DNA samples cross. This is termed as_____