Invertase is immobilised in ion-exchange resin of average diameter 1 mm. The amount of enzyme in the beads is measured by protein assay as 0.05 kg m ^-3. 20 cm ^3 beads are packed into a small column reactor; 75 ml sucrose solution at a concentration of 16 mM is pumped rapidly through the bed. In another reactor an identical quantity of free enzyme is mixed into the same volume of sucrose solution. Assume the kinetic parameters for free and immobilised enzyme are equal: K,,, is 8.8 mM and the turnover number is 2.4 x 10 gmol glucose (g enzyme)-'s^-1. The effective diffusivity of sucrose in the ion-exchange resin is 2 x 10^-6cm2s 4. What is the rate of reaction by free enzyme? 

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#Question id: 15867

#Section 6: Plant, Animal and Microbial Biotechnology

Which of the following culture is used for the production of primary and secondary metabolites?

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#Question id: 15868

#Section 6: Plant, Animal and Microbial Biotechnology

If the culture is kept in stationary phase for a prolonged period, the cells may die. Why?

a) due to the time taken for doubling of cell number

b) duration of a cell generation is too large, depending mainly on the plant species.

c) stationary phase is forced on the culture by a depletion of the nutrients

d) due to an accumulation of cellular wastes

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#Question id: 15869

#Section 6: Plant, Animal and Microbial Biotechnology

In a modest laboratory, aseptic handling, maintenance and observation of cultures, these activities can be done in the culture room.  Some facilities are required for the culture room, except?

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#Question id: 15870

#Section 6: Plant, Animal and Microbial Biotechnology

 What is the advantage of plant tissue culture over animal tissue culture?

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#Question id: 15871

#Section 6: Plant, Animal and Microbial Biotechnology

Various sterilization techniques used in plant tissue culture work;

Sterilization technique	Materials sterilized
A) Dry heat	i) Media, culture vessels, other glass plasticwarc, contaminated cultures
B) Autoclaving	ii) Heat labile compounds like GA3, ABA, zeatin, urea,·enzymes, etc.
C) Surface sterilization	iii) Empty glassware, instruments like scalpels, forceps, needles
D) Liquid (membrane filter of         0.45 μm or smaller pore size)	iv) All plant materials to be cultured

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#Question id: 15872

#Section 6: Plant, Animal and Microbial Biotechnology

For optimum callus growth, the given some following vitamins are required, except;