Invertase is immobilised in ion-exchange resin of average diameter 1 mm. The amount of enzyme in the beads is measured by protein assay as 0.05 kg m ^-3. 20 cm ^3 beads are packed into a small column reactor; 75 ml sucrose solution at a concentration of 16 mM is pumped rapidly through the bed. In another reactor an identical quantity of free enzyme is mixed into the same volume of sucrose solution. Assume the kinetic parameters for free and immobilised enzyme are equal: K,,, is 8.8 mM and the turnover number is 2.4 x 10 gmol glucose (g enzyme)-'s^-1. The effective diffusivity of sucrose in the ion-exchange resin is 2 x 10^-6cm2s 4. What is the rate of reaction by free enzyme? 

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#Question id: 14281

#Section 5: Bioprocess Engineering and Process Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 

if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 

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#Question id: 14282

#Section 5: Bioprocess Engineering and Process Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the

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#Question id: 14283

#Section 5: Bioprocess Engineering and Process Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

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#Question id: 14284

#Section 5: Bioprocess Engineering and Process Biotechnology

The specific productivity (qp) of an enzyme production Is fitted linearly with specific growth rate p of a fungal organism according to the equation The estimated values of constants alpha and beta are 0.0006 and 25 respectively. The enzyme production kinetics is 

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#Question id: 14285

#Section 5: Bioprocess Engineering and Process Biotechnology

Match the transport process with appropriate time constants given below 

Transport Process	Time Constants
1. Oxygen transfer	p. V/Q
2. Heat transfer	q. 4V(1.5ND3)
3. Flow	r. VpCp/UA
4. Mixing	s. 1/KLa

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#Question id: 14286

#Section 5: Bioprocess Engineering and Process Biotechnology

 When a liquid of density 1200kg/m3 and viscosity 0.01 kg m^-1s^-1 flows through a 1 cm ID pipe at 0.2m/s, the flow is referred as a