Baby hamster kidney cells are immobilised in alginate beads. The average particle diameter is 5 mm. Rate of oxygen consumption at a bulk concentration of 8 x 10 ^-3 kg 0 2 m ^-3 is 8.4 x 10 ^-5 kg s^- 1 m^-3 catalyst. The effective diffusivity of oxygen in the beads is 1.88 x 10^ -9 m 2 s^- 1. Assume that the oxygen concentration at the surface of the catalyst is equal to the bulk concentration, and that oxygen uptake follows zero-order kinetics. calculate the observable Thiele modulus. 

TLS Online TPP Program

#Question id: 14215

#Section 5: Bioprocess Engineering and Process Biotechnology

kLa is measured in dynamic method of oxygen transfer by 

TLS Online TPP Program

#Question id: 14216

#Section 5: Bioprocess Engineering and Process Biotechnology

The viscosity of fluid decreases with increasing stirrer speed, the fluid would be 

TLS Online TPP Program

#Question id: 14217

#Section 5: Bioprocess Engineering and Process Biotechnology

In a microbial system how true yields are related to apparent growth yield? 

TLS Online TPP Program

#Question id: 14218

#Section 5: Bioprocess Engineering and Process Biotechnology

In CSTR system at steady rate  

TLS Online TPP Program

#Question id: 14219

#Section 5: Bioprocess Engineering and Process Biotechnology

A waste-water stream of F = 1 m3 /h with substrate at 2000 mg/l is treated in an upflow packed bed containing immobilized bacteria in form of biofilm on small ceramic particles. The effluent substrate level is desired to be 30 mg/l. The rate of substrate removal is given by the following equation

                   

By using the following information, determine the required height of the column (H). k = 0.5 h-1, X = 10 g/l, Ks = 200 mg/l, L = 0.2 mm, α = 100 m2 /m3 , A = 4 m2 , η = 0.8

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TLS Online TPP Program

#Question id: 14220

#Section 5: Bioprocess Engineering and Process Biotechnology

A value of kLa = 30 h^-1 has been determined for a fermenter at its maximum practical agitator rotational speed and with air being sparged at 0.5 l gas/l reactor volume-min. E. coli with a qO2 of 10 mmol O2/g-dry wt-h are to be cultured. The critical dissolved oxygen concentration is 0.2 mg/l. The solubility of oxygen from air in the fermentation broth is 7.3 mg/l at 30⁰C.

What maximum concentration of E. coli can be sustained in this fermenter under aerobic conditions?