A strain of Azotobacter vinelandii is cultured in a 15-m³ stirred fermenter for alginate produc- tion. Under current operating conditions, k_La is 0.17 s^-1. The solubility of oxygen in the broth is approximately 8 × 10^-3 kg m^-3.

(b) The bacteria suffer growth inhibition after copper sulphate is accidentally added to the fermentation broth just after the start of the culture. This causes a reduction in the oxygen uptake rate to 3 mmol g^-1 h^-1. What maximum cell concentration can now be supported by oxygen transfer in the fermenter?.....g/l

TLS Online TPP Program

#Question id: 19486

#Section 4: Fundamentals of Biological Engineering

The growth of S.cerevisiae on glucose under anaerobic conditions can be described by the following reaction:

C₆H₁₂O₆ + aNH₃ → 0.59 CH_1.64N_0.16O_0.52 (biomass) + 0.43 C₃H₈O₃ + 1.54CO₂ + 1.3C₂H₅OH + 0.036H₂O 

and determine the product yield coefficient Y etoh/s and determine coefficient a.

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#Question id: 19487

#Section 4: Fundamentals of Biological Engineering

What is the yield coefficient if the initial substrate concentration is 20 g/l and biomass is 0.2 g/l. The substrate is then consumed and produces 6.0 g/l of biomass?

TLS Online TPP Program

#Question id: 19488

#Section 4: Fundamentals of Biological Engineering

What is the yield coefficient if the initial substrate concentration is 10 g/l and biomass is 0.1 g/l. The substrate is then consumed and produces 5.3 g/l of biomass?

TLS Online TPP Program

#Question id: 19489

#Section 4: Fundamentals of Biological Engineering

The yield coefficient is not used in growth kinetic relationship of which of the following growth kinetics?

TLS Online TPP Program

#Question id: 19530

#Section 4: Fundamentals of Biological Engineering

If air consists of 77% by weight of nitrogen and 23% by weight of oxygen calculate:

(i) the mean molecular weight of air,

(ii) the mole fraction of oxygen,

(iii) the concentration of oxygen in mole/m3 and kg/m3  if the total pressure is 1.5 atmospheres and the temperature is 25 °C.

TLS Online TPP Program

#Question id: 19531

#Section 4: Fundamentals of Biological Engineering

According to the Balances on steady-state processes, the accumulation is equal to?