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TLS Online TPP Program

#Question id: 27314

A feature of RNA that adds to its propensity to form double-helical structures is additional non-Watson–Crick base pairs. One such example is the G:U base pair, which has

#Section 3: Genetics, Cellular and Molecular Biology

  1. hydrogen bonds between N1 of uracil and the carbonyl on C6 of guanine, and between the carbonyl on C2 of uracil and N1 of guanine.

  2.  hydrogen bonds between N3 of uracil and the carbonyl on C6 of guanine, and between the carbonyl on C6 of uracil and N7 of guanine. 
  3. hydrogen bonds between N3 of uracil and the carbonyl on C6 of guanine, and between the carbonyl on C2 of uracil and N1 of guanine.
  4. hydrogen bonds between N3 of uracil and the carbonyl on C6 of guanine, and between the carbonyl on C2 of uracil and N7 of guanine. 
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TLS Online TPP Program

#Question id: 13073

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Restriction enzymes must be use in RFLP and RAPD markers for genome mapping in plants;
TLS Online TPP Program

#Question id: 13074

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Type of probe used in RFLP;
TLS Online TPP Program

#Question id: 13075

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Radioisotopes must be used in RFLP and RAPD markers
TLS Online TPP Program

#Question id: 13076

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
TLS Online TPP Program

#Question id: 13077

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The DNA probes for RFLP may NOT be obtained from
TLS Online TPP Program

#Question id: 13078

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

RFLPs have several unique advantages except this one;