Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 5232

#SCPH28 | Zoology

Using molecular clock, it was estimated that two species X and Y must have diverged from their common ancestor about 5 x 10^6 years ago. If the rate of divergence per base pair is estimated to be 0.06 per million years, what is the proportion of base pairs that differ between the two species now?

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#Question id: 19706

#SCPH01 Biochemistry

In second-degree heart block

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#Question id: 12939

#SCPH01 Biochemistry

Choose the incorrect statement for the production of site-directed mutations.

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#Question id: 2068

#I Life Science/ Life Sciences Group – I-V

Which of the following molecules dramatically increases the rate of diffusion of water across cell membranes?

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#Question id: 1121

#SCPH01 Biochemistry

Which of the following provides the best evidence that cell-signaling pathways evolved early in the history of life?