You are a scientist who is using genomics to currently study a new bacterial species that no one has ever studied before. The following sequence is a piece of DNA within the coding region of a gene that you have recently sequenced.

 

How many of the 6 potential open reading frames are actually open in this sequence shown above?

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#Question id: 18217

#SCPH05 I Biotechnology

Which of the following is untrue about homology modeling?

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#Question id: 4808

#SCPH28 | Zoology

When F1 female Drosophila of the genotype AaBbCc is test crossed, ( Gene A and B located at 10 map unit in transe configuration but gene C are independently assort for both gene. Which of the following test cross progeny that is that is more frequent?

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#Question id: 924

#SCPH01 Biochemistry

Which of the following is not true regarding the oxidation of 1 mol of palmitate (16:0) by the B-oxidation pathway?

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#Question id: 18390

#SCPH06 I Botany

Which bacterium is mainly found at quite high ionizing radiation zones or areas?

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#Question id: 13087

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?