Nurturing Life Sciences
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#Unit 12. Applied Biology
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Passaging, is the removal of the medium and transfer of cells from a previous culture into fresh growth medium, a procedure that enables the further propagation of the cell line or cell strain.
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Characteristic Growth Pattern of Cultured Cells
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Trypsin is a serine protease that digest the extracellular protein or matrix protein so that cells get free.
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Dissociation of Adherent Cells
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To check the cell viability in cell suspension, trypan blue dye is used. This dye is excluded by viable cells but taken up by dead cells (nonviable cells).