Upon secretion, Wnt proteins associate with glypicans (heparan sulfate proteoglycan) in the ECM, which restricts diffusion and leads to a greater accumulation of Wnt closer to the source of production.

TLS Online TPP Program

#Id: 4667

#Unit 13. Methods in Biology

Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. 

If all antibodies have the same affinity, then a Scatchard plot yields a straight line with a slope of Ka. The x intercept is n, the valency of the antibody, which is 2 for IgG and other divalent Igs. For IgM, which is pentameric, n  10, and for dimeric IgA, n  4. In this graph, antibody #1 has a higher affinity than antibody #2. .  

TLS Online TPP Program

#Id: 7439

#Unit 5. Developmental Biology

Fruiting structure consists of a tapered column of stalk cells that carries a spherical mass of spores aloft.

TLS Online TPP Program

#Id: 6676

#Unit 3. Fundamental Processes

Prp5 is critical for U2 binding

Brr2 facilitates U1 and U4 release

Prp2 is responsible for the activation of the spliceosome Prp22 helps the release of the mature mRNA from the spliceosome

TLS Online TPP Program

#Id: 2772

#Unit 13. Methods in Biology

DMS footprinting follows a similar principle, except that we use the DNA methylating agent DMS, instead of DNase, to attack the DNA–protein complex. 

TLS Online TPP Program

#Id: 2289

#Unit 3. Fundamental Processes

higher temperature favors tighter binding between RNA polymerase holoenzyme and T7 DNA