Neutral selection (Ka/Ks = 1)

Positive selection (Ka/Ks > 1)

Purifying selection (Ka/Ks < 1)

TLS Online TPP Program

#Id: 739

#Unit 3. Fundamental Processes

DNA strand synthesis requires the four deoxynucleoside triphosphates—dGTP, dCTP, dATP, and dTTP in equal in vivo concentration

TLS Online TPP Program

#Id: 740

#Unit 3. Fundamental Processes

primer:template junction is most important substrate for new DNA synthesis

TLS Online TPP Program

#Id: 741

#Unit 3. Fundamental Processes

The primer is complementary to, but shorter than, the template and have growing 3’ prime terminus

TLS Online TPP Program

#Id: 742

#Unit 3. Fundamental Processes

The phosphodiester bond is formed in an SN2 reaction in which the hydroxyl group at the 3’ end of the primer strand attacks the a-phosphoryl group of the incoming nucleoside triphosphate.

TLS Online TPP Program

#Id: 743

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 744

#Unit 3. Fundamental Processes

free energy for this reaction is rather small (DG–3.5 kcal/mol).