Pulsed-Field Gel Electrophoresis are used to separate very large fragment of DNA 2 × 103 kb, Essentially, this allows the separation of whole chromosomes by electrophoresis.

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#Unit 1. Molecules and their Interaction Relevant to Biology

FBPase-1 is a gluconeogenic enzyme

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#Id: 333

#Unit 1. Molecules and their Interaction Relevant to Biology

Fructose 2,6-bisphosphate binds to its allosteric site on PFK-1, it increases the enzyme’s affinity for its substrate fructose 6-phosphate and reduces its affinity for the allosteric inhibitors ATP and citrate

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#Id: 335

#Unit 1. Molecules and their Interaction Relevant to Biology

Glucagon lowers the cellular level of fructose 2,6 bisphosphate, inhibiting glycolysis and stimulating gluconeogenesis.

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#Id: 342

#Unit 1. Molecules and their Interaction Relevant to Biology

Accumulation of alanine, which can be synthesized from pyruvate in one step, allosterically inhibits pyruvate kinase, slowing the production of pyruvate by glycolysis

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#Id: 344

#Unit 1. Molecules and their Interaction Relevant to Biology

The liver PK isozyme (L form) is regulated by glucagon

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#Id: 346

#Unit 1. Molecules and their Interaction Relevant to Biology

Pyruvate Carboxylase

a mitochondrial enzyme that requires the coenzyme biotin, converts the pyruvate to oxaloacetate