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In case of nested PCR, the target sequence is amplified using a pair of PCR primers, and a portion of the amplification product is reamplified using another pair of PCR primers complementary to the regions located immediately beyond the 3'-ends of the first pair of primers.



#Unit 13. Methods in Biology #PCR #Part B Pointers
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#Unit 2. Cellular Organization

Rb binds the activation domains of a E2F transcription factors, in the cytoplasm to prevent them from turning on the genes required for cell cycle progression.

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#Unit 4. Cell Communication and Cell Signaling

Benzo(a)pyrene
(found in cigarette smoke & coal tar) undergoes metabolic activation by cytochrome P-450 in the lungs to form a potent mutagen

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#Unit 2. Cellular Organization


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#Unit 10. Ecological Principles

Larger populations are more resistant to extinctions.  Greater ranges of habitats promote increased speciation rates.


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#Unit 5. Developmental Biology

The daughter cell attached to the hub is maintained as a stem cell, whereas the cell that is not touching the hub becomes a gonialblast, a committed progenitor cell that will divide to become the precursors of the sperm cells.

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