In case of hotstart PCR, After the reaction mixture is prepared, primers can pair to the single-stranded regions in a nonspecific manner. 

A more attractive alternative is to cover the reaction mixture with a plug of inert wax and place the critical reagent on top of this plug. 

TLS Online TPP Program

#Id: 6937

#Unit 8. Inheritance Biology

 Pericentric inversion Chromosome inversion that includes the centromere in the inverted region.

TLS Online TPP Program

#Id: 5508

#Unit 10. Ecological Principles

                                                                                                                                               

TLS Online TPP Program

#Id: 692

#Unit 3. Fundamental Processes

Mutants that used to analyse Replication Machinery are usually Conditional Lethals

TLS Online TPP Program

#Id: 7503

#Unit 4. Cell Communication and Cell Signaling

RAG1 is expressed at all phases of the cell cycle, RAG2 is stable only in G0- or G1-phase cells.

TLS Online TPP Program

#Id: 851

#Unit 2. Cellular Organization

Quorum sensing in E. carotovora is mediated by N‐acyl homo serine lactones,