Selectable reporter genes

Selectable marker genes include those conferring resistance to methotrexate (dhfr, isolated from Tn7 or plasmid R67); the antibiotics kanamycin (nptII, encoding neomycin phosphotransfcrase) and hygromycin (hptIV, encoding hygromycin phosphotransferase, .isolated from E. coli);

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Bacterial phytochrome is an example of a two-component signaling system in which phytochrome functions as a sensor protein that phosphorylates a response regulator.

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Plant phytochrome is an autophosphorylating serine/threonine kinase that may phosphorylate other proteins (X).

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#Unit 6. System Physiology – Plant

The gene that encodes the MYB-related protein is therefore probably a primary response gene, and the LHCB gene itself is probably a secondary response gene.

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MYB-related protein, now known as circadian clock associated 1 (CCA1), also plays a role in the circadian regulation of LHCB gene expression.

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Second but distinct MYB-related protein, late elongated hypocotyl (LHY), has also been identified as a potential clock gene.

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When the CCA1 gene is mutated so that no functional protein is produced, circadian and phytochrome regulation of four genes, including LHCB, is affected.