Recovery of proteins using (6His-Tag) and (Ni-NTA) chromatography columns.

TLS Online TPP Program

#Id: 437

#Unit 2. Cellular Organization

One turn around the nucleosome takes 80 bp of DNA

TLS Online TPP Program

#Id: 438

#Unit 2. Cellular Organization

Two points separated by 80 bp in the free double helix may actually be one on another on nucleosome surface.

 

TLS Online TPP Program

#Id: 439

#Unit 2. Cellular Organization

H1 is most basic and heaviest

TLS Online TPP Program

#Id: 440

#Unit 2. Cellular Organization

Runs of two or more AT base pairs facilitate the bending of DNA, while runs of two or more GC base pairs have the opposite effect. 

 

TLS Online TPP Program

#Id: 441

#Unit 2. Cellular Organization

When spaced at about, consecutive AT base pair has 10 bp intervals as consecutive GC base pairs they are offset by 5 bp from runs of AT base pairs.

TLS Online TPP Program

#Id: 442

#Unit 2. Cellular Organization

The nucleosome have 200 bp of DNA with octameric basic proteins into a beadlike structure.