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TLS Online TPP Program

#Id: 3285

Eukaryotic cells also repair mismatches and do so using homologs to MutS (called MSH proteins for “MutS homologs”) and MutL (called MLH and PMS

#Unit 3. Fundamental Processes #DNA damage and repair mechanisms #Part B Pointers
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TLS Online TPP Program

#Id: 561

#Unit 2. Cellular Organization

lacZ encodes for b-galactosidase, a tetramer of 500 kD. The enzyme breaks lactose is cleaved into glucose and galactose. This enzyme also produces an important byproduct, b-1,6-allolactase.
TLS Online TPP Program

#Id: 562

#Unit 2. Cellular Organization

The lac mRNA (as most mRNA is in bacteria) is extremely unstable and decays with a half-life of only 3 minutes.
TLS Online TPP Program

#Id: 563

#Unit 2. Cellular Organization

B-1,6-allolactase is a true inducer of Lac operon which activates Lac ZYA gene expression by inhibiting Lac Repressor
TLS Online TPP Program

#Id: 564

#Unit 2. Cellular Organization

Negative Regulation/ Positive Regulation or catabolic repression
TLS Online TPP Program

#Id: 565

#Unit 2. Cellular Organization

Molecules that induce enzyme synthesis but are not metabolized are called gratuitous inducers such as IPTG
TLS Online TPP Program

#Id: 566

#Unit 2. Cellular Organization

IPTG (isopropyl b-D-1-thiogalactopyranoside) X-gal (5-bromo4-chloro-3-indolyl-b-D-galactopyranoside). When acted on by that enzyme, X-gal releases a blue color that makes it useful as substrate in assays.