Agglutination reactions are similar in principle to precipitation reactions they depend on the crosslinking of polyvalent antigens. Just as an excess of antibody inhibits precipitation reactions, such excess can also inhibit agglutination reactions; this inhibition is called the prozone effect.

TLS Online TPP Program

#Id: 8615

#Unit 2. Cellular Organization

Y15 & T14 are situated in the G-loop of the CDK, containing the consensus sequence GEGT14Y15GVV

TLS Online TPP Program

#Id: 8616

#Unit 2. Cellular Organization

Phosphorylation of T14 and Y15 in this G-loop

introduces negative charge that electrostatically interferes with the binding of ATP in the catalytic pocket.

TLS Online TPP Program

#Id: 8617

#Unit 2. Cellular Organization

Wee1 kinase Inhibits CDKs in all organisms

TLS Online TPP Program

#Id: 8618

#Unit 2. Cellular Organization

Cdc25 phosphatase Activates CDKs in fission yeast

TLS Online TPP Program

#Id: 8619

#Unit 2. Cellular Organization

INK4s

bind to CDK4 and CDK6 monomers, blocking their interaction with cyclin D

TLS Online TPP Program

#Id: 8620

#Unit 2. Cellular Organization

A second class of CKIs found in metazoan cells is the Cip/Kip family of CKIs, consists of three proteins—p21CIP, p27KIP1, and p57KIP2.