Cdc25 phosphatase Activates CDKs in fission yeast

TLS Online TPP Program

#Id: 873

#Unit 13. Methods in Biology

Variants of PCR has been devised for mutational analysis AS-PCR, Site-directed-mutagenesis (Oligonucleotide-directed Mutagenesis), overlap extension PCR, Megaprimer PCR.

TLS Online TPP Program

#Id: 874

#Unit 13. Methods in Biology

Touchdown PCR, has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature.

TLS Online TPP Program

#Id: 875

#Unit 13. Methods in Biology

HOT START PCR were devised to prevent non – specific amplification at low temperature.

TLS Online TPP Program

#Id: 876

#Unit 13. Methods in Biology

FRET is possible only when the distance between donor and acceptor are within 1 to 10 Å

TLS Online TPP Program

#Id: 877

#Unit 13. Methods in Biology

GFP chromophore comprises an oxidized form of the tripeptide –Ser65–Tyr66–Gly67–.

TLS Online TPP Program

#Id: 878

#Unit 13. Methods in Biology

The efficiency of FRET is inversely proportional to the sixth power of the distance between donor and acceptor.