Nurturing Life Sciences
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#Unit 3. Fundamental Processes
An arrested RNA polymerase can restart transcription by cleaving the RNA transcript to generate a new 3' end.
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RNA polymerase performs two proofreading functions on that growing transcript
pyrophosphorolytic editing
hydrolytic editing
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In the Pyrophosphorolytic editing, the enzyme uses its active site, in a simple back-reaction, to catalyze the removal of an incorrectly inserted ribonucleotide, by reincorporation of PPi.
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Hydrolytic editing, is the polymerase backtracks by one or more nucleotides and cleaves the RNA product, removing the error-containing sequence.
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Hydrolytic editing is stimulated by Gre factors, which both enhance hydrolytic editing function and serve as elongation stimulating factors.
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The Nus proteins—joins polymerase in the elongation phase and promotes.