TLS Online TPP Program

#Id: 4725

#Unit 13. Methods in Biology

Destructive method uses Folin reagent, Biuret, BCA, Coomassie Brilliant Blue and give absorbance at 660, 540, 562, 595 nm of wavelength respectively.

TLS Online TPP Program

#Id: 4726

#Unit 13. Methods in Biology

The constructive method involved direct analysis of protein and gives absorbance at wavelength of 280 nm.

TLS Online TPP Program

#Id: 4727

#Unit 13. Methods in Biology

Carotenoids gives absorbance between 400 – 500 nm of wavelength while porphyrin gives absorbance at 400 nm of wavelength known as Soret band.

TLS Online TPP Program

#Id: 4728

#Unit 13. Methods in Biology

DNA gives absorbance at 595 nm in presence of Diphenylamine while gives direct absorbance at 260 nm.

TLS Online TPP Program

#Id: 4729

#Unit 13. Methods in Biology

RNA gives absorbance at 595 nm in presence of Diphenylamine while gives directabsorbance at 540 nm.

TLS Online TPP Program

#Id: 4730

#Unit 13. Methods in Biology

The characteristic absorption parameter for the sample is the extinction coefficient a, yielding the correlation I= I 0 e -d .