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#Id: 2506

#Unit 13. Methods in Biology

In the Isoelectric Focusing due to the pH gradient (e.g. pH 3–10) positive charged protein are separated near pH 10 while negatively amino acid are separated near pH 3.

TLS Online TPP Program

#Id: 2507

#Unit 13. Methods in Biology

Two-Dimensional Polyacrylamide Gel Electrophoresis  technique combines the technique of IEF (first dimension), which separates proteins in a mixture according to charge (pI), with the size separation technique of SDS–PAGE (second dimension).

TLS Online TPP Program

#Id: 2508

#Unit 13. Methods in Biology

Cellulose Acetate Electrophoresis have clinical role for example enzyme and antibodies are easily detected by using Cellulose Acetate Electrophoresis with zymogen technique.

TLS Online TPP Program

#Id: 2509

#Unit 13. Methods in Biology

The most commonly used general protein stain for detecting protein on gels is the sulfated trimethylamine dye Coomassie Brilliant Blue R-250 (CBB).

TLS Online TPP Program

#Id: 2510

#Unit 13. Methods in Biology

Silver staining gives higher sensitivity comparison to Coomassie Brilliant Blue (CBB).

TLS Online TPP Program

#Id: 2511

#Unit 13. Methods in Biology

Glycoproteins have traditionally been detected on protein gels by use of the periodic acid–Schiff ( PAS) stain