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Five ways to splice an RNA


#Unit 3. Fundamental Processes #Capping, Polyadenylation, Splicing and Alternative splicing #Part B Pointers
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TLS Online TPP Program

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#Unit 13. Methods in Biology

Propidium iodide-labeled cells in order as


Apoptotic cells


G1/G0


S


G2/M  








TLS Online TPP Program

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#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).


TLS Online TPP Program

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#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 


TLS Online TPP Program

#Id: 4657

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles


TLS Online TPP Program

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#Unit 13. Methods in Biology

CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.


TLS Online TPP Program

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#Unit 13. Methods in Biology

The 51Cr Release Assay is used to measure Cell death not apoptosis



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