TLS Online TPP Program

#Id: 2752

#Unit 13. Methods in Biology

AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

TLS Online TPP Program

#Id: 2753

#Unit 13. Methods in Biology

TLS Online TPP Program

#Id: 2754

#Unit 13. Methods in Biology

An AFLP is detected as a differential mobility of an amplified DNA fragment; AFLPs, therefore, behave as codominant markers during inheritance.

TLS Online TPP Program

#Id: 2755

#Unit 13. Methods in Biology

Linker and/or adapter molecules can be joined to the cut ends. Linkers are short, chemically synthesized, double-stranded oligonucleotides, which contain within them one or more restriction endonuclease sites, e.g., linker 5'CCGAATTCGG (only one strand of the linker is shown here) contains one EcoRI site. 

TLS Online TPP Program

#Id: 2756

#Unit 13. Methods in Biology

Linkers are fused with blunt-ended DNA fragments; cleavage of the linker with the appropriate restriction enzyme creates suitable cohesive protruding ends.

TLS Online TPP Program

#Id: 2757

#Unit 13. Methods in Biology

Linkers have the following two applications: creation of cohesive 'ends

on blunt~ended DNA fragments, and 

on fragments having unmatched or undefined sequences in their protruding ends.