Coronatine is a toxin effector produced by the bacterium Pseudomonas syringae to mimic the plant hormone jasmonic acid‐isoleucine and this assists bacterial infection.

TLS Online TPP Program

#Id: 806

#Unit 3. Fundamental Processes

Hinkle and Chamberlin experiment showed that the polymerase holoenzyme binds much more tightly to the T7 DNA than does the core enzyme.

TLS Online TPP Program

#Id: 807

#Unit 3. Fundamental Processes

Hinkle and Chamberlin experiment also showed that the melted DNA has higher affinity for holoenzyme

TLS Online TPP Program

#Id: 808

#Unit 3. Fundamental Processes

A promoter is defined by the presence of short consensus sequences at specific locations.

TLS Online TPP Program

#Id: 809

#Unit 3. Fundamental Processes

In the bacterial genome, the minimum length of promoter that could provide an adequate signal is 12 bp.  The minimum length required for unique recognition increases with the size of genome, a problem in eukaryotic genomes.

TLS Online TPP Program

#Id: 810

#Unit 3. Fundamental Processes

The 12-bp promoter sequence need not be contiguous. 

If a specific number of base pairs separates two constant shorter sequences, their combined length could be less than 12 bp, because the distance of separation itself provides a part of the signal (usually 16-17 bp)

TLS Online TPP Program

#Id: 811

#Unit 3. Fundamental Processes