TLS Online TPP Program

#Question id: 910


Experiments on denaturation and renaturation after the reduction and reoxidation of the —S—S— bonds in the enzyme ribonuclease (RNase) have shown that:

#Part-B Specialized Branches in Biotechnology
  1. folding of denatured RNase into the native, active conformation, requires the input of energy in the form of heat.

  2. native ribonuclease does not have a unique secondary and tertiary structure.

  3. the completely unfolded enzyme, with all —S—S— bonds broken, is still enzymatically active.

  4. the primary sequence of RNase is sufficient to determine its specific secondary and tertiary structure.