TLS Online TPP Program

#Question id: 150


How do the pKa values of an ionizable side chain compare when the amino acid is free versus when it is in a polypeptide chain?

#Section 2: General Biology
  1. The pKa of the side chain is independent of whether the amino acid is in a polypeptide chain or is free.

  2. The pKa of the side chain is always lower for the free amino acid.

  3. The pKa of the side chain may be lower or higher in a polypeptide chain due to weaker inductive effects and differences in their microenvironments.

  4. The pKa of the side chain is usually higher in a polypeptide chain due to stabilization from nearby residues in the three-dimensional structure.

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TLS Online TPP Program

#Question id: 13103

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.
 
 

Why is it that some people show only one band?

TLS Online TPP Program

#Question id: 13104

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.
 

 
Why is it that some people show two bands?

TLS Online TPP Program

#Question id: 13105

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.
  

Given the data so far, for which of the following statement is correct

TLS Online TPP Program

#Question id: 13106

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 
 
Why is it that some people only have one band?

TLS Online TPP Program

#Question id: 13107

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.
 

 
Why is it that some people show two bands?

TLS Online TPP Program

#Question id: 13108

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 
 Why is it that some people show three bands?