Choose the correct order for the cloning process.

1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.

2. mRNA cannot be used directly in cloning.

3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.

4. The cDNA may be inserted into vectors and then cloned.

TLS Online TPP Program

#Question id: 15478

#Section 6: Plant, Animal and Microbial Biotechnology

Escherichia coli preferentially utilizes glucose by preventing the uptake of lactose and switching off transcription of the lac operon. This mechanism is known as 

TLS Online TPP Program

#Question id: 15479

#Section 6: Plant, Animal and Microbial Biotechnology

Out of the following what usually supports the fastest growth rate and is therefore considered the preferred nitrogen source for E. coli

TLS Online TPP Program

#Question id: 15480

#Section 6: Plant, Animal and Microbial Biotechnology

Feedback repression involves the turning “off” of enzyme synthesis when the amount of the product has been made in sufficient quantities. The end product of the pathway acts as

TLS Online TPP Program

#Question id: 15481

#Section 6: Plant, Animal and Microbial Biotechnology

One of the approaches for the bacterial strain improvement for primary metabolite include

TLS Online TPP Program

#Question id: 15482

#Section 6: Plant, Animal and Microbial Biotechnology

Over expression of primary metabolites are often achieved by

1. Frequency of RNA polymerase binding to the promoter region

2. Copy number increase of structural genes for these enzymes by genetic engineering

3. Chemically derived protein

Which combination below represent correct option?

TLS Online TPP Program

#Question id: 15483

#Section 6: Plant, Animal and Microbial Biotechnology

A novel technique for strain improvement combining the advantage of multi-parental crossing allowed by DNA shuffling with the recombination of entire genomes.