Choose the correct order for the cloning process.

1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.

2. mRNA cannot be used directly in cloning.

3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.

4. The cDNA may be inserted into vectors and then cloned.

#Question id: 1124

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following is (are) intracellular second messengers?

a. Ca2+                        b. cAMP          c. epinephrine             

d. protein kinase A      e. protein kinase C

#Question id: 1125

#Section 3: Genetics, Cellular and Molecular Biology

Examples of G protein–coupled receptors include

a. rhodopsin receptor.             b. β-adrenergic receptor.

c. insulin receptor.                   d. acetylcholine receptor.

#Question id: 1126

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following statement(s) is (are) true of the α subunit of a G protein?

a. It physically associates with the receptor.              

b. It binds GDP.

c. It binds GTP.                     

d. It is a transmembrane protein.

#Question id: 1127

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following steps does not occur after acetylcholine binds to the muscarinic acetylcholine receptor in heart muscle cells?

#Question id: 1128

#Section 3: Genetics, Cellular and Molecular Biology

During the epinephrine stimulated conversion of glycogen to glucose 1-phosphate, which of the following steps involves protein phosphorylation?

a. inactivation of glycogen synthase               b. activation of protein kinase A

c. activation of adenylyl cyclise                      d. activation of glycogen phosphorylase

#Question id: 1129

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following plays a role in the relaxation of smooth muscle cells by nitric oxide?

a. cGMP          b. protein kinase A      c. protein kinase G      d. guanylyl cyclise