The function f(x) = e^x -1 is to be solved using Newton-Raphson method. If the initial value of xo is taken as 1.0, then the absolute error observed at 2nd iteration is . 

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#Question id: 21163

#Section 5: Bioprocess Engineering and Process Biotechnology

The half life period of a first order reaction is given by (where, K = rate constant. )

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#Question id: 13088

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

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#Question id: 3341

#Section 3: Genetics, Cellular and Molecular Biology

In a given population of 400 individuals, 64 percent of population is caused  cancer due to completely dominat allele B. Assuming the population is in Hardy- Weinberg equilibrium, which of the following is the expected proportion of individuals who are not expected to develop the cancer?

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#Question id: 3635

#Section 3: Genetics, Cellular and Molecular Biology

In the multiple allele series that determines coat color in rabbits, c+codes for agouti, cch for chinchilla and ch for Himalayan. Dominance is in the order c+>cch>ch. In a cross of c+/cch X cch/ch, what proportion of progeny will be chinchilla?

TLS Online TPP Program

#Question id: 21726

#Section 5: Bioprocess Engineering and Process Biotechnology

For a first order chemical reaction, the rate constant