The curvature of the crista junctions is due to a protein complex called MICOS (mitochondrial contact site and cristae organizing system).

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#XL - Q Biochemistry

The curvature of the crista junctions is due to a protein complex called MICOS (mitochondrial contact site and cristae organizing system).

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#XL - T Zoology

In unstimulated cells, 

Raf is phosphorylated by several cytosolic kinases on two sites:

1. Serine/threonine kinase domain 

2. N-terminal autoinhibitory domain

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Molecular Handcuff 

Phosphorylation of Raf is recognize by protein 14-3-3 dimer, which locks the autoinhibitory domain in a position where it blocks and inhibits the kinase active site

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#XL - T Zoology

TLS Online TPP Program

#Id: 9645

#XL - T Zoology

Active Ras⋅GTP binds to the N-terminal autoinhibitory domain of Raf, altering its conformation and allowing the phosphate attached to this domain to be removed by cytosolic phosphatases, causing loss of 14-3-3 binding.

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#XL - T Zoology

Active Raf phosphorylates MEK on one or two serine residues in its activation loop, activating MEK kinase activity

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#XL - T Zoology

Constitutively active Raf kinase

a glutamic acid substitution for the valine at position 600