LHCII, one of the membrane-bound antenna pigment proteins, which can be phosphorylated and non-phosphorylated by a specific kinase present in thylakoid membrane. Which of the following statements are NOT TRUE ?

#Question id: 4349

#Unit 3. Fundamental Processes

RNA Pol IV and Pol V

#Question id: 4122

#Unit 3. Fundamental Processes

RNA Pol uses its active site, in a simple back-reaction, to catalyze the removal of an incorrectly inserted ribonucleotide. This phenomenon known as

#Question id: 4121

#Unit 3. Fundamental Processes

The transition from the closed to the open complex involves structural changes in the enzyme and the opening of the DNA double helix to reveal the template and nontemplate strands. This “melting” involves

A. Isomerization reaction and it does not require energy derived from ATP hydrolysis

B. Isomerization is essentially irreversible and, once complete, typically guarantees that transcription will subsequently initiate

C. Positions –11 and -2, with respect to the transcription start site.

#Question id: 4120

#Unit 3. Fundamental Processes

Which of the following homolog matching is incorrectly matched with thier respective subunits?

#Question id: 4119

#Unit 3. Fundamental Processes

Many eukaryotic genes contain a large number of exons. Correct splicing of such genes requires that neighboring exons be ligated to one another; if they are not, exons will be left out. One early proposal suggested that the splicing machinery bound to a splice site at one end of an intron and scanned through the intron to find the splice site at the other end. Such a scanning mechanism would guarantee that an exon was never skipped. This hypothesis was tested with one minigene with a duplicated 5ʹ splice site. Find diagram of the products you expect from minigene if the splicing machinery binds to a 5ʹ splice site and scans toward a 3ʹ splice site.

#Question id: 4118

#Unit 3. Fundamental Processes

If the 5’ splice site sequence changed from 5’- GUAAGU-3’ to 5’-GUAUGU-3’, predict the effect of the sequence change on U1 binding and U6 snRNP binding in an in vitro protein– RNA binding assay.