GRB2 SH2 domain binds to a specific phosphotyrosine residue in the cytosolic domain of an activated RTK.

 

TLS Online TPP Program

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#Unit 4. Cell Communication and Cell Signaling

Binding of Notch to Delta 

is followed by endocytosis of Delta by the signaling cell

TLS Online TPP Program

#Id: 10051

#Unit 4. Cell Communication and Cell Signaling

The force accompanying the movement of Delta into the signaling cell stretches the Notch protein on the responding cell and allowing access by ADAM 10, which cleaves the Notch extracellular domain

TLS Online TPP Program

#Id: 10052

#Unit 4. Cell Communication and Cell Signaling

Second cleavage of Notch occurs within membrane-spanning region & catalyzed by γ-secretase

TLS Online TPP Program

#Id: 10053

#Unit 4. Cell Communication and Cell Signaling

γ-secretase complex contains 

Presenilin 1 (actual protease), aph-1, pen-2, & nicastrin

TLS Online TPP Program

#Id: 10054

#Unit 4. Cell Communication and Cell Signaling

Cleavage by the γ-secretase complex releases the Notch cytosolic segment, which translocates to the nucleus, interacts with a single DNA-binding transcription factor, CSL.

TLS Online TPP Program

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#Unit 4. Cell Communication and Cell Signaling

Growth factors and EGFs are synthesized as TM proteins, regulated cleavage of the precursor by members of the ADAM protease releases the active molecule into the extracellular space.